tbe buffer (Biotium)
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tbe buffer
Tbe Buffer, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 608 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbe+buffer/TBE+Buffer/pm42885290-110-25-57
Average 96 stars, based on 608 article reviews
Tbe Buffer, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 608 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbe+buffer/TBE+Buffer/pm42885290-110-25-57
Average 96 stars, based on 608 article reviews
tbe buffer - by Bioz Stars,
2026-11
96/100 stars
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Polymerase Chain Reaction:Article Title: Assessment of Genetic Diversity and Population Structure in Oil-Bearing Rose Genotypes Using Start Codon-Targeted (SCoT) Markers Article Snippet: PCR amplifications were performed in a final volume of 20 μL containing 1 μL genomic DNA (20 ng), 10 μL Red Taq DNA Polymerase 2× Master Mix (1.5 mM MgCl ), 1 μL primer (10 pmol), and 8 μL ddH O. .. All PCR reactions were carried out using a Doppio Gradient 2 × 48-well thermal cycler (VWR ® , Darmstadt, Germany) under the following conditions: initial denaturation at 94 °C for 5 min; 35 cycles of denaturation at 94 °C for 45 s, primer annealing at 50–58 °C for 45 s, and extension at 72 °C for 90 s; followed by a final extension at 72 °C for 10 min. PCR products were visualized on 1.7% agarose gels prepared in 1× Article Title: Assessment of Genetic Diversity and Population Structure in Oil-Bearing Rose Genotypes Using Start Codon-Targeted (SCoT) Markers Article Snippet: PCR amplifications were performed in a final volume of 20 μL containing 1 μL genomic DNA (20 ng), 10 μL Red Taq DNA Polymerase 2× Master Mix (1.5 mM MgCl2), 1 μL primer (10 pmol), and 8 μL ddH2O. .. All PCR reactions were carried out using a Doppio Gradient 2 × 48-well thermal cycler (VWR®, Darmstadt, Germany) under the following conditions: initial denaturation at 94 ◦C for 5 min; 35 cycles of denaturation at 94 ◦C for 45 s, primer annealing at 50–58 ◦C for 45 s, and extension at 72 ◦C for 90 s; followed by a final extension at 72 ◦C for 10 min. PCR products were visualized on 1.7% agarose gels prepared in 1× Article Title: Epigallocatechin gallate and thermal cycling‑stimulation synergistically promote apoptosis in A549 cells via endoplasmic reticulum stress‑induced calcium ion dysregulation and oxidative stress. Article Snippet: .. To achieve optimal resolution among the unspliced (XBP1u), heteroduplex (XBP1h) and spliced (XBP1s) isoforms, PCR products were separated on a 2.5% agarose gel prepared in Staining:Article Title: Assessment of Genetic Diversity and Population Structure in Oil-Bearing Rose Genotypes Using Start Codon-Targeted (SCoT) Markers Article Snippet: PCR amplifications were performed in a final volume of 20 μL containing 1 μL genomic DNA (20 ng), 10 μL Red Taq DNA Polymerase 2× Master Mix (1.5 mM MgCl ), 1 μL primer (10 pmol), and 8 μL ddH O. .. All PCR reactions were carried out using a Doppio Gradient 2 × 48-well thermal cycler (VWR ® , Darmstadt, Germany) under the following conditions: initial denaturation at 94 °C for 5 min; 35 cycles of denaturation at 94 °C for 45 s, primer annealing at 50–58 °C for 45 s, and extension at 72 °C for 90 s; followed by a final extension at 72 °C for 10 min. PCR products were visualized on 1.7% agarose gels prepared in 1× Article Title: Assessment of Genetic Diversity and Population Structure in Oil-Bearing Rose Genotypes Using Start Codon-Targeted (SCoT) Markers Article Snippet: PCR amplifications were performed in a final volume of 20 μL containing 1 μL genomic DNA (20 ng), 10 μL Red Taq DNA Polymerase 2× Master Mix (1.5 mM MgCl2), 1 μL primer (10 pmol), and 8 μL ddH2O. .. All PCR reactions were carried out using a Doppio Gradient 2 × 48-well thermal cycler (VWR®, Darmstadt, Germany) under the following conditions: initial denaturation at 94 ◦C for 5 min; 35 cycles of denaturation at 94 ◦C for 45 s, primer annealing at 50–58 ◦C for 45 s, and extension at 72 ◦C for 90 s; followed by a final extension at 72 ◦C for 10 min. PCR products were visualized on 1.7% agarose gels prepared in 1× Article Title: DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout. Article Snippet: .. Electrophoresis was performed in 0.5 × Article Title: Epigallocatechin gallate and thermal cycling‑stimulation synergistically promote apoptosis in A549 cells via endoplasmic reticulum stress‑induced calcium ion dysregulation and oxidative stress. Article Snippet: .. To achieve optimal resolution among the unspliced (XBP1u), heteroduplex (XBP1h) and spliced (XBP1s) isoforms, PCR products were separated on a 2.5% agarose gel prepared in Article Title: Cryo-EM structures of NHEJ assemblies with nucleosomes Article Snippet: Binding reactions (10 mL) containing the indicated concentrations of Ku70 SAP protein and 19.3 nM of a 400 bp DNA fragment (generated by PCR using primers 5′-GAGTTTTATCGCTTCCATGAC and 5′-ACTTGACTCATGATTTCTTACC, with PhiX174 DNA (NEB) as the template) were prepared in 105 mM KCl, 3 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 mM DTT, and 4% glycerol. .. Reactions were incubated for 15 min at room temperature before fractionation on a 0.8% agarose gel (10 cm) in 0.5× Article Title: Electrochemical sensor for miRNA-34a detection: Synergistic signal amplification for robust and sensitive analysis Article Snippet: This study developed an electrochemical biosensor for the detection of miRNA-34a by integrating a synergistic signal amplification strategy that combined the electrocatalytic properties of reduced graphene oxide (rGO) with the enzymatic activity of double-stranded specific nuclease (DSN), while employing magnetic separation technology to ensure robust interference resistance.. The biosensor was constructed by electrodepositing rGO onto a glassy carbon electrode via cyclic voltammetry (CV), utilizing its electrocatalytic activity to enhance the electrochemical response.. Simultaneously, the presence of target miRNA-34a initiated a DSN-mediated enzymatic cyclic reaction, resulting in secondary signal amplification. Electrophoresis:Article Title: DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout. Article Snippet: .. Electrophoresis was performed in 0.5 × Article Title: Cryo-EM structures of NHEJ assemblies with nucleosomes Article Snippet: Binding reactions (10 mL) containing the indicated concentrations of Ku70 SAP protein and 19.3 nM of a 400 bp DNA fragment (generated by PCR using primers 5′-GAGTTTTATCGCTTCCATGAC and 5′-ACTTGACTCATGATTTCTTACC, with PhiX174 DNA (NEB) as the template) were prepared in 105 mM KCl, 3 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 mM DTT, and 4% glycerol. .. Reactions were incubated for 15 min at room temperature before fractionation on a 0.8% agarose gel (10 cm) in 0.5× Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test. Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in other:Article Title: Aging-associated endolysosomal decline drives inflammaging and neurodegeneration through the STING-IFN-I axis Article Snippet: After phenol/chloroform extraction, the aqueous supernatant was incubated with 500 μg/ml DNase-free RNase A (QIAGEN) for 30 min at 37°C, again followed by phenol/chloroform extraction. Agarose Gel Electrophoresis:Article Title: Epigallocatechin gallate and thermal cycling‑stimulation synergistically promote apoptosis in A549 cells via endoplasmic reticulum stress‑induced calcium ion dysregulation and oxidative stress. Article Snippet: .. To achieve optimal resolution among the unspliced (XBP1u), heteroduplex (XBP1h) and spliced (XBP1s) isoforms, PCR products were separated on a 2.5% agarose gel prepared in Article Title: Cryo-EM structures of NHEJ assemblies with nucleosomes Article Snippet: Binding reactions (10 mL) containing the indicated concentrations of Ku70 SAP protein and 19.3 nM of a 400 bp DNA fragment (generated by PCR using primers 5′-GAGTTTTATCGCTTCCATGAC and 5′-ACTTGACTCATGATTTCTTACC, with PhiX174 DNA (NEB) as the template) were prepared in 105 mM KCl, 3 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 mM DTT, and 4% glycerol. .. Reactions were incubated for 15 min at room temperature before fractionation on a 0.8% agarose gel (10 cm) in 0.5× Article Title: Electrochemical sensor for miRNA-34a detection: Synergistic signal amplification for robust and sensitive analysis Article Snippet: This study developed an electrochemical biosensor for the detection of miRNA-34a by integrating a synergistic signal amplification strategy that combined the electrocatalytic properties of reduced graphene oxide (rGO) with the enzymatic activity of double-stranded specific nuclease (DSN), while employing magnetic separation technology to ensure robust interference resistance.. The biosensor was constructed by electrodepositing rGO onto a glassy carbon electrode via cyclic voltammetry (CV), utilizing its electrocatalytic activity to enhance the electrochemical response.. Simultaneously, the presence of target miRNA-34a initiated a DSN-mediated enzymatic cyclic reaction, resulting in secondary signal amplification. Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test. Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in Incubation:Article Title: Cryo-EM structures of NHEJ assemblies with nucleosomes Article Snippet: Binding reactions (10 mL) containing the indicated concentrations of Ku70 SAP protein and 19.3 nM of a 400 bp DNA fragment (generated by PCR using primers 5′-GAGTTTTATCGCTTCCATGAC and 5′-ACTTGACTCATGATTTCTTACC, with PhiX174 DNA (NEB) as the template) were prepared in 105 mM KCl, 3 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 mM DTT, and 4% glycerol. .. Reactions were incubated for 15 min at room temperature before fractionation on a 0.8% agarose gel (10 cm) in 0.5× Fractionation:Article Title: Cryo-EM structures of NHEJ assemblies with nucleosomes Article Snippet: Binding reactions (10 mL) containing the indicated concentrations of Ku70 SAP protein and 19.3 nM of a 400 bp DNA fragment (generated by PCR using primers 5′-GAGTTTTATCGCTTCCATGAC and 5′-ACTTGACTCATGATTTCTTACC, with PhiX174 DNA (NEB) as the template) were prepared in 105 mM KCl, 3 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 mM DTT, and 4% glycerol. .. Reactions were incubated for 15 min at room temperature before fractionation on a 0.8% agarose gel (10 cm) in 0.5× Imaging:Article Title: Cryo-EM structures of NHEJ assemblies with nucleosomes Article Snippet: Binding reactions (10 mL) containing the indicated concentrations of Ku70 SAP protein and 19.3 nM of a 400 bp DNA fragment (generated by PCR using primers 5′-GAGTTTTATCGCTTCCATGAC and 5′-ACTTGACTCATGATTTCTTACC, with PhiX174 DNA (NEB) as the template) were prepared in 105 mM KCl, 3 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 mM DTT, and 4% glycerol. .. Reactions were incubated for 15 min at room temperature before fractionation on a 0.8% agarose gel (10 cm) in 0.5× Molecular Weight:Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test. Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in Marker:Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test. Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in |